Maragno Heloisa, Rodella Patricia, Silva Freitas Josiane da, Fernando Takase Luiz
Departamento de Morfologia e Patologia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, Brazil.
Faculdade de Farmácia, Centro Universitário da Fundação Educacional de Barretos, Barretos, Brazil; Faculdade de Ciências Farmacêuticas de Araraquara, Departamento de Fármacos e Medicamentos, Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil.
Brain Res. 2015 Jun 3;1609:72-81. doi: 10.1016/j.brainres.2015.03.017. Epub 2015 Mar 17.
Phosphatidylserine (PS) is an acidic phospholipid that is widely used as an alternative and/or complementary treatment of cognitive impairments. We hypothesize that these changes may be attributable, at least in part, to alterations in hippocampal neurogenesis. The aim of the present study was to investigate the effects of acute and chronic PS administration on hippocampal cell proliferation and survival in adult (5 months old) and middle-aged (12 months old) male Wistar rats. PS was injected daily (50mg/kg, i.p.) during 7 days (acute experiment) or 21 days (chronic experiment). To label newly generated cells, rats received a single BrdU injection (200mg/kg, i.p.) one day before PS treatment. The object recognition test was performed, and the rats were perfused. The brains were removed and processed with immunohistochemistry techniques for Ki-67 (cell proliferation) and BrdU (cell survival). The acute and chronic regimens were unable to promote cognitive improvement in either age group in the object recognition test. The analysis of cell proliferation showed a significant increase in the number of Ki-67-positive cells after acute and chronic PS administration in both age groups. The analysis of cell survival showed that acute and chronic PS administration increased the number of BrdU-positive cells only in adult animals.
磷脂酰丝氨酸（PS）是一种酸性磷脂，被广泛用作认知障碍的替代和/或补充治疗方法。我们推测这些变化可能至少部分归因于海马神经发生的改变。本研究的目的是调查急性和慢性给予PS对成年（5个月大）和中年（12个月大）雄性Wistar大鼠海马细胞增殖和存活的影响。在7天（急性实验）或21天（慢性实验）期间每天腹腔注射PS（50mg/kg）。为了标记新生成的细胞，在PS治疗前一天给大鼠单次腹腔注射BrdU（200mg/kg）。进行物体识别测试，然后对大鼠进行灌注。取出大脑，用免疫组织化学技术检测Ki-67（细胞增殖）和BrdU（细胞存活）。在物体识别测试中，急性和慢性给药方案均未能促进任何一个年龄组的认知改善。细胞增殖分析显示，在两个年龄组中，急性和慢性给予PS后Ki-67阳性细胞数量均显著增加。细胞存活分析表明，急性和慢性给予PS仅在成年动物中增加了BrdU阳性细胞的数量。
