Soler Quiles Carla, Mañes Jordi, Savino Angelo, Soriano José M
Unidad Mixta de Investigación en Endocrinología, Nutrición y Dietética Clínica, Universitat de València-Instituto de Investigación Sanitaria La Fe, Valencia, España..
Departamento de Medicina Preventiva, Facultat de Farmacia, Universitat de València, España..
Nutr Hosp. 2015 Apr 1;31(4):1795-802. doi: 10.3305/nh.2015.31.4.8287.
To propose a new reference of microbiological environment monitoring in foodservice establishments.
The present work shows the determination and evaluation of the microbiological contamination generated in a foodservice establishment Method: It is based on surface sampling (microbial build-up) using mixed cellulose ester membrane filters and on air sampling (hourly microbial adhesion) using Petri dishes.
Limits of contamination are established before and during the food elaboration, by means of the microbiological analysis of the environment, surfaces and equipment systems, until reliable limits and levels of acceptance are established of each selected point. Finally, a program of environmental microbiological monitoring was established including the evaluation of all parameters that compose and are implicit in the area, thus assuring and supporting its continuity with the documentation and registers developed for a safety area. Samples for microbiological examination were collected over a period of one moth on ten different days, at two different times. Twelve selected points having previously been identified as hazardous were monitored. Furthermore, foods though to be of high risk were periodically collected for microbiological analysis.
The possibility to use of an ample range of selective media, well over the limited number used in this study, allows the analysis of many single microbial species.
提出餐饮服务场所微生物环境监测的新参考标准。
本研究展示了餐饮服务场所微生物污染的测定与评估方法。方法：基于使用混合纤维素酯膜过滤器进行表面采样（微生物累积）以及使用培养皿进行空气采样（每小时微生物附着）。
通过对环境、表面和设备系统的微生物分析，在食品加工前和加工过程中确定污染限度，直至为每个选定的点确定可靠的限度和可接受水平。最后，制定了环境微生物监测计划，包括对该区域所有构成参数和隐含参数的评估，从而通过为安全区域编制的文件和记录确保并支持其连续性。在一个月内的十个不同日子、两个不同时间收集微生物检验样本。对先前确定为危险区域的十二个选定地点进行监测。此外，定期收集被认为高风险的食品进行微生物分析。
使用比本研究中使用的有限数量更多的多种选择性培养基进行分析，能够分析许多单一微生物物种。
