Department of Chemical and Biomolecular Engineering & The Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA.
Department of Chemical and Biomolecular Engineering & The Delaware Biotechnology Institute, University of Delaware, 15 Innovation Way, Newark, DE 19711, USA; Department of Biological Sciences, University of Delaware, USA.
Curr Opin Biotechnol. 2015 Jun;33:165-75. doi: 10.1016/j.copbio.2015.01.007. Epub 2015 Mar 19.
Synthetic methylotrophy is the development of non-native methylotrophs that can utilize methane and methanol as sole carbon and energy sources or as co-substrates with carbohydrates to produce metabolites as biofuels and chemicals. The availability of methane (from natural gas) and its oxidation product, methanol, has been increasing, while prices have been decreasing, thus rendering them as attractive fermentation substrates. As they are more reduced than most carbohydrates, methane and methanol, as co-substrates, can enhance the yields of biologically produced metabolites. Here we discuss synthetic biology and metabolic engineering strategies based on the native biology of aerobic methylotrophs for developing synthetic strains grown on methanol, with Escherichia coli as the prototype.
人工合成甲基营养型是指开发非天然的甲基营养型微生物，使其能够利用甲烷和甲醇作为唯一的碳源和能源，或者与碳水化合物作为共底物来生产生物燃料和化学品等代谢产物。甲烷（来自天然气）及其氧化产物甲醇的供应不断增加，而价格却在下降，因此它们成为了有吸引力的发酵底物。由于它们比大多数碳水化合物更具还原性，因此甲烷和甲醇作为共底物可以提高生物合成代谢产物的产量。在这里，我们讨论了基于好氧甲基营养型微生物的天然生物学的合成生物学和代谢工程策略，以开发利用甲醇生长的合成菌株，以大肠杆菌为原型。
