Gan Yamei, Meng Xin, Gao Cong, Song Wei, Liu Liming, Chen Xiulai
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
Eng Microbiol. 2023 Mar 4;3(3):100081. doi: 10.1016/j.engmic.2023.100081. eCollection 2023 Sep.
The increasing shortage of fossil resources and environmental pollution has renewed interest in the synthesis of value-added biochemicals from methanol. However, most of native or synthetic methylotrophs are unable to assimilate methanol at a sufficient rate to produce biochemicals. Thus, the performance of methylotrophs still needs to be optimized to meet the demands of industrial applications. In this review, we provide an in-depth discussion on the properties of natural and synthetic methylotrophs, and summarize the natural and synthetic methanol assimilation pathways. Further, we discuss metabolic engineering strategies for enabling microbial utilization of methanol for the bioproduction of value-added chemicals. Finally, we highlight the potential of microbial engineering for methanol assimilation and offer guidance for achieving a low-carbon footprint for the biosynthesis of chemicals.
化石资源日益短缺和环境污染重新激发了人们从甲醇合成高附加值生物化学品的兴趣。然而，大多数天然或合成甲基营养菌无法以足够的速率同化甲醇来生产生物化学品。因此，甲基营养菌的性能仍需优化以满足工业应用的需求。在本综述中，我们深入讨论了天然和合成甲基营养菌的特性，并总结了天然和合成甲醇同化途径。此外，我们讨论了使微生物利用甲醇生物生产高附加值化学品的代谢工程策略。最后，我们强调了微生物工程在甲醇同化方面的潜力，并为实现化学品生物合成的低碳足迹提供指导。
