Zhang Xu, Huang Kai, Ye Yong-Jun, Shi Jun-You, Zhang Zhong-Feng
Central South University of Forestry and Technology, Changsha, China.
Central South University of Forestry and Technology, Changsha, China / Jiangmen Polytechnic, Jiangmen, China.
Pak J Pharm Sci. 2015 Mar;28(2 Suppl):761-4.
Extractives, important compounds from wood, provide abundant resources for woody medicine. In this study, the three extractives from Cunninghamia lanceolata wood were removed by method of three-stage extraction with alcohol, petroleum ether, and alcohol/petroleum ether and their chemical components were analyzed by gas chromatography-mass spectrometry (GC-MS). Thirteen chemical components were discovered in the first-stage extractives, including: 4-((1e)-3-hydroxy-1-propenyl)-2-methoxyphenol (36.80%), α-(2-phenylethenyl)-1-piperidineacetonitrile (15.39%). One-hundred chemical components were discovered in the second-stage extractives, including: [1s-(1α,4aα,10aβ)]-1, 2,3,4,4a,9,10,10a-octahydro-1,4a- dimethyl-7-(1-methylethyl)-1- phenanthrenecar-boxylic acid (15.16%), 1,3-dimethoxy-5-[(1e)-2- phenylethenyl]-benzene (6.99%). Seven chemical components were discovered in the third-stage extractives, including: 1,3-dimethoxy -5-[(1E)-2-phenylethenyl]-benzene (32.88%), stigmasta-4,6,22-trien-3α-ol (17.83%). And both the main retention time of the first-stage and which of third-stage extractives are 20-30 minutes, and the main retention time of the second-stage extractives is <10 minutes. Besides, the three extractives contained many biomedical molecular, such as [1s-(1α,4aα,10aβ)]-1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-(1-methylethyl)-1-phenanthrenecar-boxylic acid, squalene, stigmast-4-en-3-one and γ-sitosterol and so on, which means that the three extractives from Cunninghamia lanceolata wood have huge potential in biomedicine.
提取物是木材中的重要化合物，为木本药材提供了丰富的资源。在本研究中，采用乙醇、石油醚和乙醇/石油醚三级萃取法去除了杉木木材中的三种提取物，并通过气相色谱-质谱联用仪（GC-MS）分析了它们的化学成分。在一级提取物中发现了13种化学成分，包括：4-((1E)-3-羟基-1-丙烯基)-2-甲氧基苯酚（36.80%）、α-(2-苯乙烯基)-1-哌啶乙腈（15.39%）。在二级提取物中发现了100种化学成分，包括：[1S-(1α,4aα,10aβ)]-1,2,3,4,4a,9,10,10a-八氢-1,4a-二甲基-7-(1-甲基乙基)-1-菲羧酸（15.16%）、1,3-二甲氧基-5-[(1E)-2-苯乙烯基]-苯（6.99%）。在三级提取物中发现了7种化学成分，包括：1,3-二甲氧基-5-[(1E)-2-苯乙烯基]-苯（32.88%）、豆甾-4,6,22-三烯-3α-醇（17.83%）。并且一级提取物和三级提取物的主要保留时间为20-30分钟，二级提取物的主要保留时间小于10分钟。此外，这三种提取物含有许多生物医学分子，如[1S-(1α,4aα,10aβ)]-1,2,3,4,4a,9,10,10a-八氢-1,4a-二甲基-7-(1-甲基乙基)-1-菲羧酸、角鲨烯、豆甾-4-烯-3-酮和γ-谷甾醇等，这意味着杉木木材中的三种提取物在生物医学方面具有巨大潜力。
