氯离子对巴西橡胶树（橡胶树）胶乳液泡膜H⁺转运ATP酶的刺激作用。一种双重机制。
Chloride-ion stimulation of the tonoplast H+-translocating ATPase from Hevea brasiliensis (rubber tree) latex. A dual mechanism.
作者信息
Marin B P, Gidrol X
出版信息
Biochem J. 1985 Feb 15;226(1):85-94. doi: 10.1042/bj2260085.
The effect of Cl- and other anions on the tonoplast H+-translocating ATPase (H+-ATPase) from Hevea brasiliensis (rubber tree) latex was investigated. Cl- and other anions stimulated the ATPase activity of tightly sealed vesicles prepared from Hevea tonoplast, with the following decreasing order of effectiveness: Cl- greater than Br- greater than SO4(2-) greater than NO3-. As indicated by the changes of the protonmotive potential difference, anion stimulation of tonoplast H+-ATPase was caused in part by the ability of these anions to dissipate the electrical potential. This interpretation assumes not a channelling of these anions against a membrane potential, negative-inside, but a modification of the permeability of these ions through the tonoplast membrane. In addition, Cl- and the other anions stimulated the ATPase activity solubilized from the tonoplast membrane. Consequently, the tonoplast H+-pumping ATPase can be considered as an anion-stimulated enzyme. These results are discussed in relation to various models described in the literature for the microsomal H+-ATPase systems claimed as tonoplast entities.
研究了氯离子（Cl⁻）和其他阴离子对巴西橡胶树（橡胶树）乳胶液泡膜H⁺转运ATP酶（H⁺-ATP酶）的影响。Cl⁻和其他阴离子刺激了从橡胶树液泡膜制备的紧密密封囊泡的ATP酶活性，其有效性顺序如下：Cl⁻＞Br⁻＞SO₄²⁻＞NO₃⁻。正如质子动力势差的变化所示，液泡膜H⁺-ATP酶的阴离子刺激部分是由这些阴离子消散电势的能力引起的。这种解释假定这些阴离子不是逆着膜电位（内膜为负）进行通道运输，而是通过液泡膜改变这些离子的通透性。此外，Cl⁻和其他阴离子刺激了从液泡膜溶解的ATP酶活性。因此，液泡膜H⁺泵ATP酶可被视为一种阴离子刺激酶。结合文献中描述的各种被认为是液泡膜实体的微粒体H⁺-ATP酶系统模型，对这些结果进行了讨论。
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