Prasher D, McCann R O, Cormier M J
Biochem Biophys Res Commun. 1985 Feb 15;126(3):1259-68. doi: 10.1016/0006-291x(85)90321-3.
Aequorin is a bioluminescent protein which consists of a polypeptide chain (apoaequorin), coelenterate luciferin, and bound oxygen. Aequorin produces blue light upon binding Ca2+. We have isolated six recombinant pBR322 plasmids which contain apoaequorin cDNA sequences. A mixed synthetic pBR322 plasmids which contain apoaequorin cDNA sequences. A mixed synthetic oligonucleotide probe was used to identify these cDNAs. An extract of an E. coli strain possessing the largest cDNA contained apoaequorin. This apoaequorin can be converted to aequorin in the presence of coelenterate luciferin, 2-mercaptoethanol, and O2. This cDNA is therefore apparently full-length.
水母发光蛋白是一种生物发光蛋白，由一条多肽链（脱辅基水母发光蛋白）、腔肠动物荧光素和结合氧组成。水母发光蛋白在结合Ca2+时产生蓝光。我们分离出了六个含有脱辅基水母发光蛋白cDNA序列的重组pBR322质粒。一种含有脱辅基水母发光蛋白cDNA序列的混合合成pBR322质粒。使用一种混合合成寡核苷酸探针来鉴定这些cDNA。含有最大cDNA的大肠杆菌菌株提取物中含有脱辅基水母发光蛋白。在腔肠动物荧光素、2-巯基乙醇和O2存在的情况下，这种脱辅基水母发光蛋白可以转化为水母发光蛋白。因此，这个cDNA显然是全长的。
