一种平滑肌细胞系中的二氢吡啶敏感性钙通道。

Dihydropyridine sensitive calcium channels in a smooth muscle cell line.

作者信息

Kongsamut S, Freedman S B, Miller R J

出版信息

Biochem Biophys Res Commun. 1985 Feb 28;127(1):71-9. doi: 10.1016/s0006-291x(85)80127-3.

Abstract

The pharmacological properties of voltage sensitive calcium channels (VSCC) were examined in a rat aortic smooth muscle cell line (A10). The inorganic VSCC blockers Co2+ and Cd2+ blocked 45Ca2+ uptake into these cells in both 5 mM K+ and 50 mM K+ (depolarizing) conditions. The organic VSCC antagonists nitrendipine, nimodipine, D-600 and diltiazem also blocked 45Ca2+ uptake at low concentrations. The relative potencies of blockade were similar to those found in intact vascular smooth muscle. The VSCC "agonist" BAY K8644 enhanced 45Ca2+ uptake and this effect could be reversed by nitrendipine. These results indicate that A10 cells possess VSCC and that these VSCC behave similarly to those in authentic smooth muscle.

摘要

在大鼠主动脉平滑肌细胞系（A10）中研究了电压敏感性钙通道（VSCC）的药理学特性。无机VSCC阻滞剂Co2+和Cd2+在5 mM K+和50 mM K+（去极化）条件下均能阻断45Ca2+进入这些细胞。有机VSCC拮抗剂尼群地平、尼莫地平、D - 600和地尔硫䓬在低浓度时也能阻断45Ca2+摄取。阻断的相对效力与在完整血管平滑肌中发现的相似。VSCC“激动剂”BAY K8644增强了45Ca2+摄取，且这种效应可被尼群地平逆转。这些结果表明A10细胞具有VSCC，且这些VSCC的行为与真正平滑肌中的VSCC相似。