Lu Jing, Liu Jin-Bo, Sheng Rong, Liu Yi, Chen An-Lei, Wei Wen-Xue
Ying Yong Sheng Tai Xue Bao. 2014 Oct;25(10):2879-84.
In order to investigate the impact of drying process on greenhouse gas emissions and denitrifying microorganisms in paddy soil, wetting-drying process was simulated in laboratory conditions. N2O flux, redox potential (Eh) were monitored and narG- and nosZ-containing denitrifiers abundances were determined by real-time PCR. N2O emission was significantly increased only 4 h after drying process began, and it was more than 6 times of continuous flooding (CF) at 24 h. In addition, narG and nosZ gene abundances were increased rapidly with the drying process, and N2O emission flux was significantly correlated with narG gene abundance (P < 0.01). Our results indicated that the narG-containing deniteifiers were the main driving microorganisms which caused the N2O emission in the short-time drought process in paddy soil.
为了研究干燥过程对稻田土壤温室气体排放和反硝化微生物的影响，在实验室条件下模拟了干湿过程。监测了N2O通量、氧化还原电位（Eh），并通过实时PCR测定了含narG和nosZ的反硝化菌丰度。干燥过程开始仅4小时后，N2O排放就显著增加，24小时时是持续淹水（CF）的6倍多。此外，随着干燥过程，narG和nosZ基因丰度迅速增加，N2O排放通量与narG基因丰度显著相关（P < 0.01）。我们的结果表明，含narG的反硝化菌是导致稻田土壤短期干旱过程中N2O排放的主要驱动微生物。
