（111）In 六价乳糖在估计肝脏储备及其在小鼠死亡率方面的阈值的特异性和准确性。
The specificity and accuracy of (111)In-hexavalent lactoside in estimating liver reserve and its threshold value for mortality in mice.
机构信息
Institute of Nuclear Energy Research, Taoyuan 325, Taiwan; Institute of Biotechnology, National Taiwan University, Taipei 100, Taiwan.
Institute of Nuclear Energy Research, Taoyuan 325, Taiwan.
出版信息
J Hepatol. 2015 Aug;63(2):370-7. doi: 10.1016/j.jhep.2015.02.052. Epub 2015 Mar 19.
BACKGROUND & AIMS: The asialoglycoprotein receptor on hepatocyte membranes recognizes the galactose residues of glycoproteins. We investigated the specificity, accuracy and threshold value of asialoglycoprotein receptor imaging for estimating liver reserve via scintigraphy using (111)In-hexavalent lactoside in mouse models.
METHODS
(111)In-hexavalent lactoside scintigraphy for asialoglycoprotein receptor imaging was performed on groups of normal mice, orthotopic SK-HEP-1-bearing mice, subcutaneous HepG2-bearing mice, mice with 20-80% partial hepatectomy and mice with acute hepatitis induced by acetaminophen. Liver reserve was measured by relative liver uptake and compared with normal mice. Asialoglycoprotein receptor blockade was performed via an in vivo asialofetuin competitive binding assay.
RESULTS
A total of 73.64±7.11% of the injection dose accumulated in the normal liver tissue region, and radioactivity was barely detected in the hepatoma region. When asialoglycoprotein receptor was blocked using asialofetuin, less than 0.41±0.04% of the injection dose was detected as background in the liver. Asialoglycoprotein receptor imaging data revealed a linear correlation between (111)In-hexavalent lactoside binding and residual liver mass (R(2)=0.8548) in 20-80% of partially hepatectomized mice, demonstrating the accuracy of (111)In-hexavalent lactoside imaging for measuring the functional liver mass. Asialoglycoprotein receptor imaging data in mice with liver failure induced using 600mg/kg acetaminophen revealed 19-45% liver reserve relative to normal mice and a fatal threshold value of 25% liver reserve.
CONCLUSION
The (111)In-hexavalent lactoside imaging method appears to be a good, specific, visual and quantitative predictor of functional liver reserve. The diagnostic threshold for survival was at 25% liver reserve in mice.
背景与目的
肝细胞膜上的去唾液酸糖蛋白受体识别糖蛋白中的半乳糖残基。我们通过使用（111）In-六乳糖对小鼠模型进行闪烁扫描，研究了去唾液酸糖蛋白受体成像对估计肝储备的特异性、准确性和临界值。
方法
对正常小鼠、原位 SK-HEP-1 荷瘤小鼠、皮下 HepG2 荷瘤小鼠、行 20%-80%部分肝切除术的小鼠和用对乙酰氨基酚诱导急性肝炎的小鼠进行（111）In-六乳糖闪烁扫描以进行去唾液酸糖蛋白受体成像。通过相对肝摄取测量肝储备，并与正常小鼠进行比较。通过体内去唾液酸胎球蛋白竞争结合测定进行去唾液酸糖蛋白受体阻断。
结果
正常肝组织区域共累积了注射剂量的 73.64±7.11%，而肝癌区域几乎检测不到放射性。当使用去唾液酸胎球蛋白阻断去唾液酸糖蛋白受体时，肝内检测到的注射剂量不到 0.41±0.04%作为背景。去唾液酸糖蛋白受体成像数据显示，20%-80%部分肝切除小鼠中（111）In-六乳糖结合与剩余肝质量之间呈线性相关（R²=0.8548），表明（111）In-六乳糖成像用于测量功能性肝质量的准确性。用 600mg/kg 对乙酰氨基酚诱导肝衰竭的小鼠的去唾液酸糖蛋白受体成像数据显示，相对于正常小鼠，肝储备为 19%-45%，并存在 25%肝储备的致死临界值。
结论
（111）In-六乳糖成像方法似乎是一种良好、特异、直观和定量的功能性肝储备预测方法。小鼠存活的诊断阈值为 25%的肝储备。
Zotero 插件