Navrotskaya V, Oxenkrug G, Vorobyova L, Sharma H, Muresanu D, Summergrad P
Department of Genetics and Cytology, V. N. Karazin Kharkiv National University, Kharkiv, Ukraine.
Neuroinflammation Program, Department of Psychiatry, Tufts University/Tufts Medical Center, Boston, MA, USA.
Am J Neuroprot Neuroregen. 2014 Oct 1;6(1):65-68. doi: 10.1166/ajnn.2014.1083.
Cerebrolysin® (CBL) is a neuroprotective drug used for the treatment of neurodegenerative diseases. CBL's mechanisms of action remain unclear. Involvement of tryptophan (TRP)-kynurenine (KYN) pathway in neuroprotective effect of CBL might be suggested considering that modulation of KYN pathway of TRP metabolism by CBL, and protection against eclosion defect and prolongation of life span of Drosophila melanogaster with pharmacologically or genetically-induced down-regulation of TRP conversion into KYN. To investigate possible involvement of TRP-KYN pathway in mechanisms of neuroprotective effect of CBL, we evaluated CBL effects on metamorphosis and life span of Drosophila melanogaster maintained at 23 °C and 28 °C ambient temperature. CBL accelerated metamorphosis, exerted strong tendency ( = 0.04) to prolong life span in female but not in male flies, and attenuated aging-accelerating effect of high (28 °C) ambient temperature in both female and male flies. Further research of CBL effects on metamorphosis and resistance to aging-accelerating effect of high temperature might offer new insights in mechanisms of its neuroprotective action and expand its clinical applications.
脑蛋白水解物（Cerebrolysin®，CBL）是一种用于治疗神经退行性疾病的神经保护药物。CBL的作用机制尚不清楚。鉴于CBL对色氨酸（TRP）代谢的犬尿氨酸（KYN）途径的调节作用，以及通过药理学或基因手段下调TRP向KYN的转化对果蝇羽化缺陷的保护作用和寿命的延长，可以推测TRP-KYN途径参与了CBL的神经保护作用。为了研究TRP-KYN途径是否可能参与CBL神经保护作用的机制，我们评估了CBL对在23℃和28℃环境温度下饲养的果蝇变态发育和寿命的影响。CBL加速了果蝇的变态发育，对雌性果蝇有延长寿命的强烈趋势（P = 0.04），但对雄性果蝇没有此作用，并且减弱了高温（28℃）对雌性和雄性果蝇加速衰老的影响。进一步研究CBL对果蝇变态发育和抗高温加速衰老作用的影响，可能会为其神经保护作用机制提供新的见解，并扩大其临床应用。
