多年生草本植物华中獐牙菜（獐牙菜科）多态微卫星位点的开发。
Development of polymorphic microsatellite loci in the perennial herb Hepatica nobilis var. japonica (Ranunculaceae).
机构信息
Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyoku, Kyoto 606-8501, Japan.
出版信息
Appl Plant Sci. 2015 Feb 18;3(3). doi: 10.3732/apps.1400114. eCollection 2015 Mar.
PREMISE OF THE STUDY
Microsatellite markers were developed and characterized in the vulnerable plant Hepatica nobilis var. japonica (Ranunculaceae) to investigate its genetic diversity, population structure, and gene flow.
METHODS AND RESULTS
Fourteen microsatellite markers were developed. The number of alleles per locus ranged from one to 12, and the expected heterozygosity per locus ranged from 0.043 to 0.855. Eleven markers were successfully amplified in the cultivar 'Mego' from Japan.
CONCLUSIONS
These microsatellite markers can be used to investigate the genetic diversity, population structure, and gene flow of H. nobilis var. japonica.
研究前提
开发并鉴定脆弱植物延龄草（毛茛科）中的微卫星标记，以研究其遗传多样性、种群结构和基因流。
方法和结果
开发了 14 个微卫星标记。每个位点的等位基因数范围从 1 到 12，每个位点的预期杂合度范围从 0.043 到 0.855。11 个标记在日本品种“Mego”中成功扩增。
结论
这些微卫星标记可用于研究延龄草（var. japonica）的遗传多样性、种群结构和基因流。
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