Glende E A, Lee P Y
Exp Mol Pathol. 1985 Apr;42(2):167-74. doi: 10.1016/0014-4800(85)90025-5.
Administration of isopropanol (2.5 ml/kg, po) or chlordecone (15.2 mg/kg, po) potentiated the release of glutamic oxaloacetic transaminase (GOT) into serum 17- or 7-fold, respectively, in rats exposed subsequently to 30 microliter CCl4/kg, po. Hepatocytes isolated from isopropanol-treated rats, incubated with low concentrations of CCl4 (0.3 or 0.9 mM), did not have significant increase in the amount of GOT released after 30 min compared to control cells exposed to CCl4. However, at 3 hr cells from isopropanol-treated rats released 10- or 3-fold more GOT when exposed to 0.3 or 0.9 mM CCl4, respectively, than control cells exposed to CCl4. By hour 5 of incubation this differential of GOT release was not observed. The same dose and time-dependent pattern of potentiated GOT release upon exposure of CCl4 was seen in hepatocytes obtained from chlordecone-treated rats. These results indicate that the potentiation by isopropanol or chlordecone of CCl4-induced release of GOT from liver is retained through the procedures of cell isolation.
给大鼠口服异丙醇（2.5毫升/千克）或开蓬（15.2毫克/千克），随后再给大鼠口服30微升/千克四氯化碳，结果显示，血清中的谷氨酸草酰乙酸转氨酶（GOT）释放量分别增强了17倍或7倍。与暴露于四氯化碳的对照细胞相比，用低浓度四氯化碳（0.3或0.9毫摩尔）培养从经异丙醇处理的大鼠分离出的肝细胞30分钟后，GOT释放量没有显著增加。然而，在3小时时，与暴露于四氯化碳的对照细胞相比，经异丙醇处理的大鼠的细胞在暴露于0.3或0.9毫摩尔四氯化碳时，GOT释放量分别多出10倍或3倍。在培养5小时时，未观察到GOT释放的这种差异。从经开蓬处理的大鼠获得的肝细胞中，也观察到了四氯化碳暴露后GOT释放增强的相同剂量和时间依赖性模式。这些结果表明，通过细胞分离程序，异丙醇或开蓬对四氯化碳诱导的肝脏GOT释放的增强作用得以保留。
