Effect of antigastrin (SC-15396) on amylase release and 2-deoxyglucose uptake in mouse pancreatic acini.

The effects of antigastrin (SC-15396) on amylase release and 2-deoxyglucose uptake were studied in dispersed acini from mouse pancreas. Antigastrin at concentrations between 0.5 and 2 mM inhibited cholecystokinin (CCK)-stimulated amylase release and 2-deoxyglucose uptake in a dose-dependent fashion. Antigastrin at concentrations between 0.5 and 2 mM inhibited carbachol-stimulated amylase release and 2-deoxyglucose uptake as well. In addition, the drug (2 mM) inhibited bombesin-stimulated amylase release and 2-deoxyglucose uptake. In contrast, the stimulation of amylase release by Ca2+ ionophore A23187 was not inhibited by antigastrin. The results, therefore, suggest that antigastrin nonselectively inhibits the actions of these secretagogues probably at their receptor sites.