Yu Hui-juan, Huang Shu-mei, Chao Hui, Ji Liang-nian
Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, P.R. China; MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
J Inorg Biochem. 2015 Aug;149:80-7. doi: 10.1016/j.jinorgbio.2015.02.020. Epub 2015 Mar 6.
Two new Ru(II) complexes Ru(tpy)(dpoq)Cl1 and Ru(tpy)(dpoq)CH3CN2 (tpy = 2,2':6',2''-terpyridine; dpoq = dipyrido[1,2,5]oxadiazolo[3,4-b]quinoxaline) have been synthesized and characterized by elemental analysis, (1)H NMR, electrospray ionization mass spectra (ESI-MS) and X-ray crystallographic study. The experimental results of spectra titration, thermal denaturation and viscosity measurements suggest that the two complexes intercalatively bind to DNA. When irradiated under light, the two complexes could efficiently photocleave DNA both under aerobic and anaerobic condition. The mechanism studies reveal that the photocleavage reaction functions through both oxygen-independent (photoinduced electron transfer, type III reaction) and oxygen-dependent (singlet oxygen generation, type II reaction) pathways and the oxygen-independent pathway is the major process. These complexes will be more promising photodynamic therapy (PDT) candidates used for treating hypoxic tumors.
合成了两种新型钌（II）配合物Ru（tpy）（dpoq）Cl1和Ru（tpy）（dpoq）CH3CN2（tpy = 2,2':6',2''-三联吡啶；dpoq = 二吡啶并[1,2,5]恶二唑并[3,4-b]喹喔啉），并通过元素分析、(1)H NMR、电喷雾电离质谱（ESI-MS）和X射线晶体学研究对其进行了表征。光谱滴定、热变性和粘度测量的实验结果表明，这两种配合物以插入方式与DNA结合。光照下，这两种配合物在有氧和无氧条件下均能有效光切割DNA。机理研究表明，光切割反应通过不依赖氧（光诱导电子转移，III型反应）和依赖氧（单线态氧生成，II型反应）的途径起作用，且不依赖氧的途径是主要过程。这些配合物将是用于治疗缺氧肿瘤的更有前景的光动力疗法（PDT）候选物。
