Babinska A, Cierniewski C S, Koziolkiewicz W, Janecka A
Int J Pept Protein Res. 1985 Jan;25(1):69-75. doi: 10.1111/j.1399-3011.1985.tb02147.x.
Synthetic fragments and analogs were used to characterize specificity of antisera to SP and SP6-11. [Tyr8] SP and [Lys6] SP6-11 were both used as radioiodinated ligands. The latter was conjugated with Bolton-Hunter reagents before labelling. In both systems, the C-terminal pentapeptide SP7-11 was the shortest fragment showing antigenic identity with Substance P molecule. Substitution of different amino acid residues in SP6-11 by His or Gly showed that all but Glu6 take part in the structure of the antigenic determinant.
