Karr R W
Hum Immunol. 1985 Mar;12(3):177-83. doi: 10.1016/0198-8859(85)90334-9.
HLA-DS molecules are the human homologues of murine I-A molecules. The molecular specificities of four anti-DS reagents were examined by two-dimensional gel electrophoresis in light of the recent evidence that HLA-DR homozygous cell lines express at least two DS molecules. Each of the DS-specific reagents, including the IVD12 monoclonal antibody which defines an MB3-like determinant, isolated at least two DS molecules from a DR5 homozygous cell line. Because previous studies of this cell line documented that anti-MB3 allosera isolated only one DS molecule, these data indicate that there is heterogeneity of the molecular specificities of anti-Ia reagents that are specific for the MB3 allodeterminant or the MB3-like determinant in population studies.
HLA-DS分子是鼠类I-A分子的人类同源物。鉴于最近有证据表明HLA-DR纯合细胞系表达至少两种DS分子，通过二维凝胶电泳检测了四种抗-DS试剂的分子特异性。每种DS特异性试剂，包括定义MB3样决定簇的IVD12单克隆抗体，都从DR5纯合细胞系中分离出至少两种DS分子。由于此前对该细胞系的研究表明抗-MB3同种异体血清仅分离出一种DS分子，这些数据表明在群体研究中，针对MB3同种异型决定簇或MB3样决定簇的抗-Ia试剂的分子特异性存在异质性。
