Vaupel M R, Sherwood O D, Anderson M B
J Histochem Cytochem. 1985 Apr;33(4):303-8. doi: 10.1177/33.4.2579996.
Immunocytochemical staining for relaxin in ovarian sections of pregnant mice from day 11 through day 18 of gestation revealed that only corpora lutea (CL) of pregnancy are stained. Evaluation of serial sections of ovaries from a day 16 pregnant mouse revealed that the only luteal structures present are CL of pregnancy. The number of CL present in each ovary equaled the number of implantation sites in each related horn (7 on the right side and 8 on the left side). These large CL varied in shape, being round in some profiles to very elongate in others. All CL were immunochemically stained for relaxin using the peroxidase-antiperoxidase method of L. Sternberger (Immunocytochemistry, 2nd ed. Wiley, New York, 1979). The intensity of the strain varied from cell to cell within each CL. Small luteal structures that were observed to be immunochemically stained for relaxin were demonstrated to represent the periphery of CL of pregnancy. No luteinized follicles were observed and interstitial cells and follicles were not immunochemically stained in any of the day 16 serial ovarian sections or in any of the ovarian sections from pregnant mice on the other days of gestation studied. CL of previous cycles were not observed to be present in the ovaries at days 15, 16, or 18 of gestation. However on day 14 and before, CL of previous cycles were observed and they did not exhibit any relaxin immunostaining. Immunocytochemical studies using the biotin-avidin system revealed that no relaxin immunostaining could be demonstrated in the ovaries of cycling mice at any stage of the estrous cycle. In conclusion, this study revealed that the only ovarian structures demonstrating relaxin immunocytochemical staining in the mouse were CL of pregnancy.
对妊娠第11天至第18天的妊娠小鼠卵巢切片进行松弛素免疫细胞化学染色，结果显示只有妊娠黄体（CL）被染色。对一只妊娠第16天小鼠的卵巢连续切片进行评估，发现存在的唯一黄体结构是妊娠CL。每个卵巢中CL的数量与每个相关子宫角的着床部位数量相等（右侧7个，左侧8个）。这些大的CL形状各异，有些轮廓呈圆形，有些则非常细长。使用L. Sternberger的过氧化物酶-抗过氧化物酶方法（《免疫细胞化学》，第2版，Wiley，纽约，1979年）对所有CL进行松弛素免疫化学染色。每个CL内细胞间染色强度各不相同。观察到的对松弛素进行免疫化学染色的小黄体结构被证明代表妊娠CL的周边。未观察到黄素化卵泡，在妊娠第16天的任何连续卵巢切片或所研究的其他妊娠天数的妊娠小鼠卵巢切片中，间质细胞和卵泡均未进行免疫化学染色。在妊娠第15、16或18天的卵巢中未观察到前一周期的CL。然而，在第14天及之前，观察到了前一周期的CL，它们未表现出任何松弛素免疫染色。使用生物素-抗生物素蛋白系统的免疫细胞化学研究表明，在发情周期的任何阶段，周期小鼠的卵巢中均未显示出松弛素免疫染色。总之，本研究表明，在小鼠中唯一显示松弛素免疫细胞化学染色的卵巢结构是妊娠CL。
