Yoshioka M, Yamaguchi K, Urano Y, Mori S
Gan No Rinsho. 1985 Jan;31(1):45-51.
Binding of 2 lectins--peanut agglutinin (PNA) and Dolichos biflorus agglutinin (DBA)--with human colonic carcinomas, adenomas, juvenile and hyperplastic polyps, and polyps in Cronkhite-Canada syndrome was examined histochemically by the lectin-antilectin-PAP method. With PNA, brush borders of the carcinoma cell were stained(90.9%), while the cytoplasm of the adenomas and polyps were stained granularly (88.0%). With DBA, the cytoplasm of the carcinomas was stained diffusely (72.7%), though 25% of the cases showed the fringed type cytoplasmic staining similar to adenomas, polyps and normal crypts. It appears important to recognize the differences in the staining patterns, especially in PNA, between carcinomas and other benign lesions, which apparently reflect changes of sugar residues during cancerization.
采用凝集素-抗凝集素-过氧化物酶抗过氧化物酶（PAP）法，对2种凝集素——花生凝集素（PNA）和双花扁豆凝集素（DBA）与人结肠癌、腺瘤、幼年性息肉、增生性息肉以及克-卡综合征（Cronkhite-Canada syndrome）中的息肉的结合情况进行了组织化学检查。PNA可使癌细胞的刷状缘染色（90.9%），而腺瘤和息肉的细胞质呈颗粒状染色（88.0%）。DBA可使癌细胞的细胞质呈弥漫性染色（72.7%），不过25%的病例显示出与腺瘤、息肉及正常隐窝相似的边缘型细胞质染色。认识到癌与其他良性病变在染色模式上的差异很重要，尤其是在PNA染色方面，这些差异显然反映了癌变过程中糖残基的变化。
