Ratti Regiane Priscila, Delforno Tiago Palladino, Okada Dagoberto Yukio, Varesche Maria Bernadete Amâncio
University of São Paulo, Brazil.
University of São Paulo, Brazil.
Microbiol Res. 2015 Apr;173:10-7. doi: 10.1016/j.micres.2015.01.010. Epub 2015 Feb 7.
In this study, the composition and diversity of the bacterial community in thermophilic H2-producing reactors fed with glucose were investigated using pyrosequencing. The H2-producing experiments in batch were conducted using 0.5 and 2.0 g l(-1) glucose at 550 °C. Under the two conditions, the H2 production and yield were 1.3 and 1.6 mol H2 mol glucose(-1), respectively. Acetic, butyric, iso-butyric, lactic and propionic acids were detected in the two reactors. The increase in substrate concentration favored a high H2 yield. In this reactor, a predominance of acetic and iso-butyric acids, 27.7% and 40%, were measured, respectively. By means of pyrosequencing, a total of 323 and 247 operational taxonomic units were obtained, with a predominance of the phylum Firmicutes (68.73-67.61%) for reactors with 0.5 and 2.0 g l(-1) glucose, respectively. Approximately 40.55% and 62.34% of sequences were affiliated with Thermoanaerobacterium and Thermohydrogenium, microorganisms that produce H2 under thermophilic conditions.
在本研究中，利用焦磷酸测序技术研究了以葡萄糖为原料的嗜热产氢反应器中细菌群落的组成和多样性。在55℃下，使用0.5和2.0 g l(-1)葡萄糖进行分批产氢实验。在这两种条件下，氢气产量和产率分别为1.3和1.6 mol H2 mol葡萄糖(-1)。在两个反应器中均检测到乙酸、丁酸、异丁酸、乳酸和丙酸。底物浓度的增加有利于提高氢气产率。在该反应器中，乙酸和异丁酸分别占主导地位，比例分别为27.7%和40%。通过焦磷酸测序，分别获得了323个和247个操作分类单元，对于分别含有0.5和2.0 g l(-1)葡萄糖的反应器，厚壁菌门占主导地位（分别为68.73-67.61%）。约40.55%和62.34%的序列隶属于嗜热厌氧杆菌属和嗜热产氢菌属，这两种微生物在嗜热条件下产氢。
