Karra Vijay Kumar, Gumma Phani Kumar, Chowdhury Soumya Jyoti, Ruttala Rajesh, Polipalli Sunil Kumar, Chakravarti Anita, Kar Premashis
PCR Hepatitis Lab, Dept. of Medicine, Maulana Azad Medical College, University of Delhi, New Delhi 110002, India.
Department of Medical Microbiology, Maulana Azad Medical College, University of Delhi, New Delhi 110002, India.
Cytokine. 2015 Jun;73(2):277-82. doi: 10.1016/j.cyto.2015.02.015. Epub 2015 Mar 21.
Interleukine-18 (IL-18) was originally called interferon (INF-γ) inducing factor and plays a critical dual role in Th1 polarization and viral clearance. We aimed to explore whether single-nucleotide promoter polymorphisms (SNPs) are associated with the outcome of hepatitis B virus (HBV) infection. 271 HBV infected patients were recruited in this study out of these 109 were spontaneously recovered and 162 were diagnosed to be having persistent HBV infection which includes 48 chronic hepatitis, 84 liver cirrhosis, 30 HCC cases and were compared with 280 healthy controls. IL-18 promoter genotyping was performed with sequence-specific primers. The results demonstrated the significant involvement of genotype AA at position -607 in healthy controls (38.6%) when compared to cases (26.0%) (OR=0.54 (0.385-0.797)) and also associated with spontaneous clearance (37.6%) compared to persistent HBV infections (17.9%) (OR=2.76 (1.582-4.832)). Whereas, genotype CC at position -607 in cases (18.0%) when compared to healthy controls (6.7%) (OR=3.03 (1.734-5.303)) also associated with persistent HBV infections (24.1%) compared to spontaneous clearance (9.2%) (OR=0.31 (0.151-0.67)). And genotype GC at position -137 in cases (49.5%) compared to healthy controls (38.5%) (OR=1.55 (1.11-2.18)). Whereas, genotype GG at position -137 in healthy controls (56.8%) compared to cases (45.4%) (OR=0.63 (0.451-0.885)). No significant difference at position -137 was observed between spontaneous clearance and persistent HBV infections. These polymorphisms of the IL-18 gene promoter region at position -607 and -137 could be associated with different outcomes of HBV infection. The people with allele A at position -607 may be protected against HBV infection; moreover AA genotype is associated with spontaneous clearance.
白细胞介素-18（IL-18）最初被称为干扰素（INF-γ）诱导因子，在Th1极化和病毒清除中发挥关键的双重作用。我们旨在探讨单核苷酸启动子多态性（SNP）是否与乙型肝炎病毒（HBV）感染的结局相关。本研究招募了271例HBV感染患者，其中109例自发康复，162例被诊断为持续性HBV感染，包括48例慢性肝炎、84例肝硬化、30例肝癌病例，并与280例健康对照进行比较。采用序列特异性引物进行IL-18启动子基因分型。结果显示，与病例组（26.0%）相比，健康对照组中-607位点的AA基因型显著更多（38.6%）（OR=0.54（0.385-0.797）），并且与自发清除组（37.6%）相比，与持续性HBV感染组（17.9%）相比也存在关联（OR=2.76（1.582-4.832））。然而，与健康对照组（6.7%）相比，病例组中-607位点的CC基因型更多（18.0%）（OR=3.03（1.734-5.303）），与持续性HBV感染组（24.1%）相比，与自发清除组（9.2%）相比也存在关联（OR=0.31（0.151-0.67））。病例组中-137位点的GC基因型（49.5%）与健康对照组（38.5%）相比（OR=1.55（1.11-2.18））。然而，健康对照组中-137位点的GG基因型（56.8%）与病例组（45.4%）相比（OR=0.63（0.451-0.885））。在自发清除组和持续性HBV感染组之间，-137位点未观察到显著差异。IL-18基因启动子区域-607和-137位点的这些多态性可能与HBV感染的不同结局相关。-607位点携带A等位基因的人可能对HBV感染具有保护作用；此外，AA基因型与自发清除相关。
