Tsertsvadze T Sh, Mitskevich N G, Ghirdaladze D M, Porakishvili N Z
Georgian Med News. 2015 Feb(239):35-8.
Chronic lymphocytic leukemia (CLL) is the most common leukaemia in the US and in Europe, including Georgia. Patients with CLL are susceptible to infectious diseases as a result of both, the disease progression and chemotherapy that indicates deficiency of immune responses to pathogens, including innate immunity, mediated by monocytes. Monocytes are also often recruited by monoclonal antibodies (mAbs) which express anti-tumour toxicity through Fcγ-receptor (FcγR)-mediated phagocytosis of opsonised leukaemic cells. In this paper we address of monocytes functional status through assessment of the patterns of expression of Fcγ receptors CD64, CD32, CD16 and CD180 receptor on monocytes from CLL patients and healthy individuals using specific mAbs and flow cytometry. Our data demonstrate that monocytes from peripheral blood of CLL patients lack expression of CD64 and CD16 as well as CD180 that would substantially undermine their ability to contribute to anti-bacterial immune responses. In addition, aberrant expression of CD64 would negatively affect the efficiency of antibody-mediated immunotherapies.
慢性淋巴细胞白血病（CLL）是美国和欧洲（包括格鲁吉亚）最常见的白血病。CLL患者由于疾病进展和化疗，对传染病易感，这表明对病原体的免疫反应存在缺陷，包括由单核细胞介导的固有免疫。单核细胞也常被单克隆抗体（mAb）募集，这些单克隆抗体通过Fcγ受体（FcγR）介导的调理素化白血病细胞吞噬作用表现出抗肿瘤毒性。在本文中，我们通过使用特异性单克隆抗体和流式细胞术评估CLL患者和健康个体单核细胞上Fcγ受体CD64、CD32、CD16和CD180受体的表达模式，来探讨单核细胞的功能状态。我们的数据表明，CLL患者外周血中的单核细胞缺乏CD64、CD16以及CD180的表达，这将严重削弱它们参与抗菌免疫反应的能力。此外，CD64的异常表达会对抗体介导的免疫治疗效率产生负面影响。
