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[用于免疫酶分析的抗免疫球蛋白过氧化物酶缀合物获取方法的研发]

[Development of a method of obtaining peroxidase conjugates with anti-immunoglobulin for immunoenzyme analysis].

作者信息

Iankina N F, Vaneeva L I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1985 Jan(1):35-40.

PMID:2580406
Abstract

The results of the successive stages of preparing peroxidase-labeled anti-immunoglobulins are presented. The schedules for the immunization of animals have been worked out with a view to prepare antisera to human gamma globulin and, subsequently, to isolate antibodies from them by different methods. The preparations obtained with the use of immunosorbent have proved to possess the highest serological activity. As the result of these investigations, a new method of binding, a modification of the glutaraldehyde method, has been proposed. The conjugates obtained by the proposed method are characterized by high serological activity, stability and good reproducibility.

摘要

本文展示了制备过氧化物酶标记抗免疫球蛋白连续阶段的结果。制定了动物免疫方案,旨在制备抗人γ球蛋白抗血清,随后通过不同方法从其中分离抗体。已证明使用免疫吸附剂获得的制剂具有最高的血清学活性。作为这些研究的结果,提出了一种新的结合方法,即戊二醛方法的改进。通过该方法获得的缀合物具有高血清学活性、稳定性和良好的重现性。

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