GDP-D-甘露糖-4,6-脱水酶检测法
An assay for GDP-D-mannose-4,6-dehydratase.
作者信息
Chang S, Broschat K O, Serif G S
出版信息
Anal Biochem. 1985 Jan;144(1):253-7. doi: 10.1016/0003-2697(85)90113-7.
PMID:2580455
Abstract
The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C5 hydrogen has been established using GDP-D-[5-3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.
摘要
利用GDP-D-[5-³H]-甘露糖作为底物，已确定了GDP-D-甘露糖-4,6-脱水酶作用于C5氢原子丢失的机制。这一观察结果已被纳入一种基于³H与水相介质平衡的该酶快速检测方法中。
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