Department of Plastic and Reconstructive Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China ; Stem Cell and Tissue Engineering Research Center, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
Department of Bioengineering, Rice University, Houston 77251-1892, TX, USA.
Neural Regen Res. 2012 Feb 5;7(4):256-60. doi: 10.3969/j.issn.1673-5374.2012.04.003.
In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.
在本研究中，从小鼠坐骨神经中分离出雪旺细胞，并用Dispase 和胶原酶进行纯化。结果表明，在用 Dispase 进行第一轮纯化后，大多数雪旺细胞呈圆形，15 分钟后漂浮在培养液中。此外，与胶原酶组相比，细胞产量和细胞纯度更高。第二轮纯化后，Dispase 组的最终细胞产量高于胶原酶组，但细胞纯度无显著差异。此外，在成年 Sprague-Dawley 大鼠中也观察到了类似的细胞数量和纯度结果。这些发现表明，Dispase 纯化可以快速分离出产量和纯度高的雪旺细胞。
