Piersma A H, Brockbank K G, Ploemacher R E, van Vliet E, Brakel-van Peer K M, Visser P J
Exp Hematol. 1985 May;13(4):237-43.
Several properties of fibroblastic colony-forming units (CFU-F) from murine bone marrow and their in vitro progeny were evaluated. CFU-F had a high buoyant density relative to total bone marrow cells; they were noncycling in situ and adhered to nylon wool. The fibroblastic cells stained positively for fibronectin, lipid, alkaline phosphatase, and nonspecific esterase, while phagocytosis assays were negative, and ultrastructural analysis failed to reveal desmosomes. These properties contrasted bone-marrow-derived fibroblastic cells to both endothelial cells and macrophages. Fibroblastic cells derived from several hemopoietic organs and skin were screened for antigenic determinants present on hemopoietic cells using monoclonal antibodies. Mac-1 and B220 were absent from all fibroblastic cells studied, whereas the Forsmann and Pgp-1 antigens were always present. Thy-1 was not detected on bone-marrow-derived fibroblasts, but was present on fibroblastic cells derived from other sources. T200 was found on all hemopoietic organ-derived fibroblastic cells, but not on those derived from blood and skin. Thus, analysis of antigenic determinants allowed distinction between fibroblastic cells from different organs.
对来自小鼠骨髓的成纤维细胞集落形成单位（CFU-F）及其体外子代的若干特性进行了评估。相对于全骨髓细胞，CFU-F具有较高的浮力密度；它们在原位处于非循环状态，并能黏附于尼龙纤维。成纤维细胞对纤连蛋白、脂质、碱性磷酸酶和非特异性酯酶呈阳性染色，而吞噬试验为阴性，超微结构分析未发现桥粒。这些特性将骨髓来源的成纤维细胞与内皮细胞和巨噬细胞区分开来。使用单克隆抗体对源自多个造血器官和皮肤的成纤维细胞进行筛选，以寻找造血细胞上存在的抗原决定簇。在所研究的所有成纤维细胞中均不存在Mac-1和B220，而福斯曼抗原和Pgp-1抗原始终存在。在骨髓来源的成纤维细胞上未检测到Thy-1，但在源自其他来源的成纤维细胞上存在。在所有造血器官来源的成纤维细胞上发现了T200，但在血液和皮肤来源的成纤维细胞上未发现。因此，对抗原决定簇的分析使得能够区分来自不同器官的成纤维细胞。
