Yuan Hai-chao, Tang Yin, Bai Yun-jin, Li Jin-hong, Pu Chun-xiao, Wang Kun-jie, Li Hong, Wei Qiang, Han Ping
Sichuan Da Xue Xue Bao Yi Xue Ban. 2015 Jan;46(1):1-5.
To investigate the feasibility of autologous bone marrow mesenchymal stem cells(BMSCs) seeded on bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model.
This study included 25 mongrel dogs. Five dogs were sacrificed for the preparation of BAMG. Twenty dogs were randomly divided into two groups and received partial cystectomy. In group A, the bladder defect was repaired with unseeded BAMG. In group B, the bladder defect was repaired with autologous BMSCs-seeded BAMG. The bladders were retrieved and studied histologically and immunohistochemically at the time point of 1, 2, 4, 8, 12 weeks after surgery to evaluate tissue regeneration.
All dogs survived the procedure. Histopathological examination in group B showed there was urothelium developed at the end of the 2 weeks. By 8 and 12 weeks all bladder wall components were regenerated in the repaired area, which were similar to normal bladder tissue. In group A, urothelium regeneration was observed at the end of the 4 weeks, whereas smooth muscle was still not well-formed by 12 weeks.
Autologous BMSCs-seeded BAMG could promote the repair of bladder defect, which is superior to unseeded BAMG in regenerative properties.
探讨自体骨髓间充质干细胞（BMSCs）接种于膀胱脱细胞基质移植物（BAMG）用于犬膀胱重建的可行性。
本研究纳入25只杂种犬。处死5只犬用于制备BAMG。将20只犬随机分为两组并接受膀胱部分切除术。A组用未接种细胞的BAMG修复膀胱缺损。B组用接种自体BMSCs的BAMG修复膀胱缺损。术后1、2、4、8、12周时取出膀胱，进行组织学和免疫组织化学研究以评估组织再生情况。
所有犬手术过程中均存活。B组组织病理学检查显示，术后2周时可见尿路上皮形成。至8周和12周时，修复区域的膀胱壁各层均再生，与正常膀胱组织相似。A组术后4周时可见尿路上皮再生，而至12周时平滑肌仍未良好形成。
接种自体BMSCs的BAMG可促进膀胱缺损修复，其再生性能优于未接种细胞的BAMG。
