Victor-Kobrin C, Bikoff E K, Bona C A
J Immunol. 1985 Jun;134(6):3686-92.
In this paper we describe the polyclonal blast response induced in nonimmune murine splenocytes by two homologous alpha 1-3 dextran-binding myeloma protein, J558 and MOPC104E. This stimulation appears to be independent of MHC or IgCh gene complex control, and proceeds entirely in a T-dependent fashion. The responding B cell population appears to belong to the more mature Lyb-5+ subset. This response was elicited by several independently prepared batches of J558 and MOPC104E, each of which was conclusively shown to be free of endotoxin contamination. Experiments are presented that suggest that this stimulation is being mediated via the Fab portion of these two myeloma proteins, in particular, a shared IdX, rather than through their Fc.
在本文中，我们描述了两种同源的α1-3葡聚糖结合骨髓瘤蛋白J558和MOPC104E在非免疫小鼠脾细胞中诱导的多克隆母细胞反应。这种刺激似乎独立于MHC或IgCh基因复合体控制，并且完全以T细胞依赖的方式进行。反应性B细胞群体似乎属于更成熟的Lyb-5+亚群。几个独立制备的J558和MOPC104E批次引发了这种反应，每一批都被确凿证明无内毒素污染。所展示的实验表明，这种刺激是通过这两种骨髓瘤蛋白的Fab部分介导的，特别是通过一个共享的独特型IdX，而不是通过它们的Fc段。
