利用声学镊子细胞术通过机械刺激提高解离的人类胚胎干细胞的存活率。
Improving survival of disassociated human embryonic stem cells by mechanical stimulation using acoustic tweezing cytometry.
作者信息
Chen Di, Sun Yubing, Deng Cheri X, Fu Jianping
机构信息
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan.
出版信息
Biophys J. 2015 Mar 24;108(6):1315-1317. doi: 10.1016/j.bpj.2015.01.033.
Abstract
Dissociation-induced apoptosis of human embryonic stem cells (hESCs) hampers their large-scale culture. Herein we leveraged the mechanosensitivity of hESCs and employed, to our knowledge, a novel technique, acoustic tweezing cytometry (ATC), for subcellular mechanical stimulation of disassociated single hESCs to improve their survival. By acoustically actuating integrin-bound microbubbles (MBs) to live cells, ATC increased the survival rate and cloning efficiency of hESCs by threefold. A positive correlation was observed between the increased hESC survival rate and total accumulative displacement of integrin-anchored MBs during ATC stimulation. ATC may serve as a promising biocompatible tool to improve hESC culture.
摘要
解离诱导的人类胚胎干细胞（hESCs）凋亡阻碍了它们的大规模培养。在此，我们利用了hESCs的机械敏感性，并采用了据我们所知的一种新技术——声镊细胞术（ATC），对解离的单个hESCs进行亚细胞机械刺激，以提高它们的存活率。通过对活细胞进行声驱动整合素结合微泡（MBs），ATC将hESCs的存活率和克隆效率提高了三倍。在ATC刺激过程中，观察到hESCs存活率的提高与整合素锚定MBs的总累积位移之间存在正相关。ATC可能是一种有前景的生物相容性工具，可用于改善hESC培养。
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