黑荆树提取物对铜绿微囊藻生长的抑制机制
Inhibitory mechanisms of Acacia mearnsii extracts on the growth of Microcystis aeruginosa.
作者信息
Liu Zhibin, Zhou Lirong, Liu Dandan, Zhu Qiyu, Chen Wenqing
机构信息
* Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
* Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China E-mail:
出版信息
Water Sci Technol. 2015;71(6):856-61. doi: 10.2166/wst.2015.038.
Our previous work revealed that Acacia mearnsii extract can inhibit the growth of Microcystis aeruginosa, the common species forming toxic cyanobacterial blooms in eutrophic freshwater. In the present study, we demonstrated that this plant extract can significantly increase cell membrane permeability and Ca²⁺/Mg²⁺-ATPase activity on the membrane. Long-term exposure to concentrations of 20 ppm A. mearnsii extract led to algal cell membrane leakage or even lysis. Comparison of expression of three photosynthesis-related genes (rbcL, psaB and psbD) in M. aeruginosa with and without plant extract treatment revealed that their expression was remarkably reduced in the presence of the extract. Down-regulation of photosynthesis-related genes could indicate the inhibition of the photosynthetic process. Thus, our results suggested that both photosynthetic systems and membranes of M. aeruginosa are potentially damaged by A. mearnsii extract.
我们之前的研究表明，黑荆树提取物能够抑制铜绿微囊藻的生长，铜绿微囊藻是富营养化淡水中形成有毒蓝藻水华的常见物种。在本研究中，我们证明了这种植物提取物能够显著增加细胞膜通透性以及膜上Ca²⁺/Mg²⁺-ATP酶的活性。长期暴露于浓度为20 ppm的黑荆树提取物中会导致藻细胞膜渗漏甚至裂解。对经植物提取物处理和未经处理的铜绿微囊藻中三个光合作用相关基因（rbcL、psaB和psbD）的表达进行比较，结果显示在提取物存在的情况下，它们的表达显著降低。光合作用相关基因的下调可能表明光合过程受到抑制。因此，我们的结果表明，黑荆树提取物可能会对铜绿微囊藻的光合系统和膜造成潜在损害。
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