Singh Namita, Prasad Priya, Kumar Praveen, Singh Laishram Chandreshwar, Das Banashree, Rastogi Sangita
a Microbiology Laboratory , National Institute of Pathology (ICMR) , New Delhi , India .
b Central Instrumentation Facility , National Institute of Pathology (ICMR) , New Delhi , India , and.
J Matern Fetal Neonatal Med. 2016 Mar;29(6):1010-5. doi: 10.3109/14767058.2015.1031741. Epub 2015 Sep 25.
The aim of the study was to characterize the expression of cyclooxygenase-2 (COX-2) and prostaglandin-E2 (PGE2) genes in spontaneously aborted tissues from women infected with Chlamydia trachomatis.
A total of 135 spontaneous aborters (Group I) and 45 induced aborters (controls; Group II) attending Obstetrics and Gynaecology Department at Safdarjung hospital (New Delhi, India), were enrolled. Polymerase chain reaction (PCR) assay was performed to detect C. trachomatis DNA in endometrial curettage tissue (ECT). Differential expression of COX-2 and PGE2 receptors at mRNA level was analysed in ECT using reverse transcription PCR and real-time PCR.
In total, 14.8% patients were diagnosed as C. trachomatis-positive in Group I whereas all control patients were C. trachomatis-negative. Qualitative expression of COX-2 (p < 0.05) and PGE2 (p < 0.0001) receptors was found increased in C. trachomatis-positive patients (Group I) in comparison to controls. Quantitative real-time PCR analysis also showed upregulation in transcript levels of both COX-2 (p < 0.002) and PGE2 (p < 0.0001) receptors in infected patients (Group I) versus Group II. COX-2 and PGE2 expression was higher (p < 0.002) in recurrent spontaneous aborters in comparison to sporadic spontaneous aborters.
Results suggest that chlamydial infection leads to upregulation of COX-2 in C. trachomatis-positive recurrent spontaneous aborters, which probably mediates increased prostaglandin synthesis.
本研究旨在描述沙眼衣原体感染女性自然流产组织中环氧化酶-2（COX-2）和前列腺素E2（PGE2）基因的表达特征。
招募了共135名自然流产者（第一组）和45名人工流产者（对照组；第二组），他们均前往印度新德里萨夫达容医院妇产科就诊。采用聚合酶链反应（PCR）检测子宫内膜刮宫组织（ECT）中的沙眼衣原体DNA。使用逆转录PCR和实时PCR分析ECT中COX-2和PGE2受体在mRNA水平的差异表达。
第一组中共有14.8%的患者被诊断为沙眼衣原体阳性，而所有对照患者均为沙眼衣原体阴性。与对照组相比，沙眼衣原体阳性患者（第一组）中COX-2（p < 0.05）和PGE2（p < 0.0001）受体的定性表达增加。定量实时PCR分析还显示，与第二组相比，感染患者（第一组）中COX-2（p < 0.002）和PGE2（p < 0.0001）受体的转录水平均上调。与偶发性自然流产者相比，复发性自然流产者中COX-2和PGE2的表达更高（p < 0.002）。
结果表明，衣原体感染导致沙眼衣原体阳性复发性自然流产者中COX-2上调，这可能介导了前列腺素合成增加。
