Lou Baisong, Zhou Xin
Department of Medical Oncology, The First Hospital of Qiqihaer City of Heilongjiang, Qiqihaer, Heilongjiang 161005, P.R. China.
Int J Mol Med. 2015 May;35(5):1369-73. doi: 10.3892/ijmm.2015.2147. Epub 2015 Mar 18.
MicroRNAs (miRNAs) can function as tumor suppressors and may provide an efficient strategy for the eradication of cancer. Specific miRNAs can be reintroduced into tumor cells to complement loss of tumor suppression activities. The aim of the present study was to develop miRNA delivery formulation using synthesized miR-451. miR-451 was detected using RT-PCR and tissues were analyzed using immunohistochemical analysis. miRNA delivery formulation proved to be effective either locally or systemically. miR-451 accumulation became evident in tumor cells and exerted an anti-proliferative function. The intravenous delivery of formulated miR-451 did not induce any deregulation in the cytokine levels and liver enzymes. Taken together, the results provide insight into the concept of the systemic delivery of synthetic mimics for tumor suppressor miR-451 and provide potential implications for miRNA therapy in clinical practice.
微小RNA（miRNA）可发挥肿瘤抑制作用，并可能为根除癌症提供一种有效策略。特定的miRNA可重新导入肿瘤细胞，以补充肿瘤抑制活性的缺失。本研究的目的是使用合成的miR-451开发miRNA递送制剂。采用逆转录聚合酶链反应（RT-PCR）检测miR-451，并通过免疫组织化学分析对组织进行分析。miRNA递送制剂在局部或全身均证明有效。miR-451在肿瘤细胞中明显蓄积，并发挥抗增殖功能。静脉注射配制好的miR-451不会引起细胞因子水平和肝酶的任何失调。综上所述，这些结果为肿瘤抑制性miR-451合成模拟物的全身递送概念提供了见解，并为miRNA疗法在临床实践中的应用提供了潜在意义。
