滑菇多糖对小鼠骨髓来源树突状细胞NF-κB信号通路的影响
Effects of Pholiota nameko polysaccharide on NF-κB pathway of murine bone marrow-derived dendritic cells.
作者信息
Li Haiping, Tao Yongqing, Zhao Pei, Huai Lihua, Zhi Dexian, Liu Jiangmei, Li Guoliang, Dang Chunlan, Xu Yufeng
机构信息
Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, PR China.
Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, PR China.
出版信息
Int J Biol Macromol. 2015;77:120-30. doi: 10.1016/j.ijbiomac.2015.03.011. Epub 2015 Mar 24.
This study investigated the effect of a polysaccharide purified from Pholiota nameko (PNPS-1) on the NF-κB signaling pathway of murine bone marrow-derived dendritic cells (BMDCs) and relevant mechanisms. The results showed that PNPS-1 could decrease the expression of maturation markers CD40 and CD80 on BMDCs. PNPS-1 also could decrease the mRNA expression of Myd88, TRAF6, TIRAP, IRAKI, IKBKB, NFKB1, NFKB2 and RelA in immature BMDCs determined by RT-PCR, and decreased the IKKβ and P65 production in BMDCs determined by Western blot, and decreased the NF-кB P65 production determined by ELISA. In addition, the effects of PNPS-1 on BMDCs were significantly impaired by treating the cells with anti-TLR2 antibody prior to PNPS-1 treatment, implying direct interaction between PNPS-1 and TLR2 on cell surface. These results indicate that PNPS-1 regulates BMDCs through TLR2 and downstream NF-кB signalings.
本研究调查了从滑子菇中纯化得到的一种多糖（PNPS-1）对小鼠骨髓来源树突状细胞（BMDCs）的NF-κB信号通路的影响及相关机制。结果显示，PNPS-1可降低BMDCs上成熟标志物CD40和CD80的表达。通过RT-PCR测定，PNPS-1还可降低未成熟BMDCs中Myd88、TRAF6、TIRAP、IRAK1、IKBKB、NFKB1、NFKB2和RelA的mRNA表达，通过蛋白质免疫印迹法测定可降低BMDCs中IKKβ和P65的产生，通过酶联免疫吸附测定法测定可降低NF-κB P65的产生。此外，在PNPS-1处理前用抗TLR2抗体处理细胞，PNPS-1对BMDCs的作用显著受损，这意味着PNPS-1与细胞表面的TLR2直接相互作用。这些结果表明，PNPS-1通过TLR2和下游NF-κB信号传导调节BMDCs。
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