Deng Yi, Wang Xin, Xue Feng, Zheng Lei, Liu Jian, Yan Feng, Xia Fan, Chen Wei
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, PR China.
School of Medical Engineering, Hefei University of Technology, Hefei 230009, PR China.
Anal Chim Acta. 2015 Apr 8;868:45-52. doi: 10.1016/j.aca.2015.02.003. Epub 2015 Feb 11.
Rapid and ultrasensitive detection of trace heavy metal mercury(II) ions (Hg(2+)) are of significant importance due to the induced serious risks for environment and human health. This presented article reports the gold nanoparticle-based dual labeling colorimetric method (Dual-COLO) for ultrasensitive and rapid detection of Hg(2+) using the specific thymine-Hg(2+)-thymine (T-Hg(2+)-T) as recognition system and the dual labeling strategy for signal amplification. Both qualitative and quantitative detections of Hg(2+) are achieved successfully in aqueous samples. More importantly, the achieved detection limit of 0.005 ng mL(-1) (0.025 nM) without any instruments is very competitive to other rapid detection methods even ICP-MS based methods. This Dual-COLO method is also applied directly for real water sample monitoring and, more importantly, applied in analysis of mercury poisoned animal tissues and body fluidic samples, indicating a potentially powerful and promising tool for environmental monitoring and food safety control.
由于痕量重金属汞离子（Hg(2+)）会给环境和人类健康带来严重风险，因此对其进行快速、超灵敏检测具有重要意义。本文报道了一种基于金纳米颗粒的双标记比色法（Dual-COLO），该方法以特异性的胸腺嘧啶-汞离子-胸腺嘧啶（T-Hg(2+)-T）作为识别系统，并采用双标记策略进行信号放大，用于超灵敏、快速检测Hg(2+)。在水性样品中成功实现了Hg(2+)的定性和定量检测。更重要的是，该方法在无需任何仪器的情况下实现了0.005 ng mL(-1)（0.025 nM）的检测限，与其他快速检测方法甚至基于电感耦合等离子体质谱（ICP-MS）的方法相比具有很强的竞争力。这种Dual-COLO方法还直接应用于实际水样监测，更重要的是，应用于汞中毒动物组织和体液样本分析，表明它是一种用于环境监测和食品安全控制的潜在强大且有前景的工具。
