Nazim Uddin Md, Jeong Jae-Kyo, Seol Jae-Won, Hur Jin, Eo Seong-Kug, Lee John-Hwa, Park Sang-Youel
Biosafety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
Oncol Rep. 2015 May;33(5):2331-6. doi: 10.3892/or.2015.3869. Epub 2015 Mar 20.
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a primary anticancer agent and a member of the tumor necrosis factor family that selectively induces apoptosis in various tumor cells, but not in normal cells. Gingerol is a major ginger component with anti-inflammatory and anti‑tumorigenic activities. Autophagy flux is the complete process of autophagy, in which the autophagosomes are lysed by lysosomes. The role of autophagy in cell death or cell survival is controversial. A549 adenocarcinoma cells are TRAIL-resistant. In the present study, we showed that treatment with TRAIL slightly induced cell death, but gingerol treatment enhanced the TRAIL-induced cell death in human lung cancer cells. The combination of gingerol and TRAIL increased accumulation of microtubule-associated protein light chain 3-II and p62, confirming the inhibited autophagy flux. Collectively, our results suggest that gingerol sensitizes human lung cancer cells to TRAIL-induced apoptosis by inhibiting the autophagy flux.
肿瘤坏死因子相关凋亡诱导配体（TRAIL）是一种主要的抗癌剂，属于肿瘤坏死因子家族成员，可选择性地诱导多种肿瘤细胞凋亡，但对正常细胞无此作用。姜辣素是生姜的主要成分，具有抗炎和抗肿瘤活性。自噬通量是自噬的完整过程，其中自噬体被溶酶体裂解。自噬在细胞死亡或细胞存活中的作用存在争议。A549腺癌细胞对TRAIL耐药。在本研究中，我们发现TRAIL处理轻微诱导细胞死亡，但姜辣素处理增强了TRAIL诱导的人肺癌细胞死亡。姜辣素与TRAIL联合使用增加了微管相关蛋白轻链3-II和p62的积累，证实了自噬通量受到抑制。总体而言，我们的结果表明，姜辣素通过抑制自噬通量使人类肺癌细胞对TRAIL诱导的凋亡敏感。
