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一种用于测定RNA切割动力学的分析方法。

An assay to determine the kinetics of RNA cleavage.

作者信息

Corcoran R, Labelle M, Czarnik A W, Breslow R

出版信息

Anal Biochem. 1985 Feb 1;144(2):563-8. doi: 10.1016/0003-2697(85)90154-x.

DOI:10.1016/0003-2697(85)90154-x
PMID:2581471
Abstract

To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using polyuridylic acid as the substrate. Samples withdrawn from the kinetic run are treated with venom exonuclease (phosphodiesterase I), and the increase in the concentration of uridine is determined by high-performance liquid chromatography. A related assay has been developed to monitor the catalyzed cleavage of the dinucleotide uridylyl(3'----5') uridine (UpU).

摘要

为了评估一些模拟核糖核酸酶的合成催化剂，已开发出一种定量测定方法，该方法可测量在聚合RNA底物中裂解的磷酸二酯键的数量。该测定涉及使用聚尿苷酸作为底物来确定裂解过程中产生的5'-寡核苷酸末端的数量。从动力学实验中取出的样品用蛇毒外切核酸酶（磷酸二酯酶I）处理，尿苷浓度的增加通过高效液相色谱法测定。已开发出一种相关的测定方法来监测二核苷酸尿苷酰（3'→5'）尿苷（UpU）的催化裂解。

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Recognition and catalysis in nucleic acid chemistry.核酸化学中的识别与催化
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On the mechanism of action of ribonucleases: dinucleotide cleavage catalyzed by imidazole and Zn2+.关于核糖核酸酶的作用机制：咪唑和Zn2+催化二核苷酸裂解
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