用于在聚丙烯酰胺凝胶中选择性检测组蛋白的银染法。

Silver staining for selective detection of histones in polyacrylamide gels.

作者信息

Tsutsui K, Kurosaki T, Tsutsui K, Nagai H, Oda T

出版信息

Anal Biochem. 1985 Apr;146(1):111-7. doi: 10.1016/0003-2697(85)90404-x.

Abstract

A simple silver-staining technique was developed for selective visualization of histones in polyacrylamide gels. The specificity of the stain was confirmed using a variety of protein mixtures and isolated histones. The staining procedure requires a relatively short time to perform (2.5-3 h), and the sensitivity to lysine-rich histones is comparable to that of the conventional Coomassie blue stain (about 0.1 microgram per band). A possible mechanism for the selective staining was deduced from a comparison with the widely used ultrasensitive silver staining.

摘要

开发了一种简单的银染技术，用于在聚丙烯酰胺凝胶中选择性可视化组蛋白。使用多种蛋白质混合物和分离的组蛋白证实了该染色剂的特异性。染色过程所需时间相对较短（2.5 - 3小时），对富含赖氨酸的组蛋白的灵敏度与传统考马斯亮蓝染色相当（每条带约0.1微克）。通过与广泛使用的超灵敏银染进行比较，推断出了选择性染色的可能机制。