Cheung J Y, Leaf A, Bonventre J V
Basic Res Cardiol. 1985;80 Suppl 1:23-9. doi: 10.1007/978-3-662-11041-6_4.
Single ventricular cells tolerant to physiological levels of calcium have been successfully isolated from adult rat heart. These cells exhibit morphological as well as functional characteristics of the intact myocardium. Under anaerobic incubations either in the absence of extracellular calcium or in the presence of 6% PEG, myocytes excluded Trypan blue despite severe derangements in biochemical functions. We suggest that Trypan blue entry may be a rather insensitive criterion of cell injury. We recommend the combination of rod-shape morphology, ATP levels and the ability to contract under external pacing as alternative simple tests of cell viability.
已成功从成年大鼠心脏中分离出对生理水平钙具有耐受性的单个心室细胞。这些细胞表现出完整心肌的形态和功能特征。在无氧培养条件下，无论是在无细胞外钙的情况下还是在存在6%聚乙二醇的情况下，尽管生化功能严重紊乱，心肌细胞仍能排斥台盼蓝。我们认为台盼蓝进入可能是细胞损伤的一个相当不敏感的标准。我们建议将杆状形态、ATP水平以及在外部起搏下收缩的能力结合起来，作为细胞活力的替代简单测试。
