Gooden M, Rintoul D, Takehana M, Takemoto L
Biochem Biophys Res Commun. 1985 Apr 30;128(2):993-9. doi: 10.1016/0006-291x(85)90145-7.
Bovine and human lens membrane, when reconstituted into lipid vesicles containing oxidized cytochrome C, will mediate the transmembrane passage of ascorbate into the vesicles, where the reduction of cytochrome C is measured spectrophotometrically. This channel forming activity is specifically inhibited by antiserum made against a synthetic octapeptide near the C-terminus of MIP26K. Together, these studies describe a direct and more sensitive assay system for measurement of channel-forming activity of MIP26K, and suggest that the C-terminus of this molecule may be particularly important in the regulation of channel formation.
牛和人晶状体膜在重构到含有氧化型细胞色素C的脂质小泡中时，会介导抗坏血酸跨膜进入小泡，在小泡中通过分光光度法测量细胞色素C的还原情况。这种通道形成活性受到针对MIP26K C端附近合成八肽产生的抗血清的特异性抑制。这些研究共同描述了一种直接且更灵敏的测定系统，用于测量MIP26K的通道形成活性，并表明该分子的C端在通道形成的调节中可能特别重要。
