DNA甲基化一致性表明复发性肝细胞癌的原发肿瘤细胞起源。
DNA methylation consistency implicates the primary tumor cell origin of recurrent hepatocellular carcinoma.
作者信息
Zhang Xiaolei, Liu Shuang, Shen Congle, Wu Yali, Zhang Ling, Chen Xiangmei, Lu Fengmin
机构信息
Department of Microbiology & Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China.
Beijing Artificial Liver Treatment & Training Center, Beijing Youan Hospital, Capital Medical University, 8 Xi Tou Tiao, Beijing 100069, China.
出版信息
Epigenomics. 2015;7(4):581-92. doi: 10.2217/epi.15.23. Epub 2015 Mar 27.
AIMS
To investigate if DNA methylation pattern assay could be a new approach to identifying the primary tumor cell origin of the recurrent hepatocellular carcinoma (HCC).
MATERIALS & METHODS: Methylation of 24 genes and expression of 22 cancer stem cell (CSC) biomarkers were quantitatively measured in 10 paired primary and recurrent HCC specimens. The HBV viral-host junctions were determined in six pairs of them with HBV infection.
RESULTS
Similar DNA methylation patterns were observed among nine of ten pairs of primary and recurrent tumors. In five of six paired specimens with HBV infection, exactly same HBV DNA integrations were identified in each paired tumors. The expression of seven CSC biomarkers increased significantly in either primary or recurrent tumor tissues.
CONCLUSION
Recurrent HCCs mostly originate from their primary tumors. Assay of DNA methylation patterns could provide a new approach to determining the origin of recurrent HCC.
目的
研究DNA甲基化模式分析是否可作为一种新方法来鉴定复发性肝细胞癌（HCC）的原发肿瘤细胞起源。
材料与方法
对10对原发性和复发性HCC标本进行24个基因的甲基化定量测定以及22个癌症干细胞（CSC）生物标志物的表达定量测定。对其中6对伴有HBV感染的标本测定HBV病毒-宿主连接情况。
结果
10对原发性和复发性肿瘤中的9对观察到相似的DNA甲基化模式。在6对伴有HBV感染的标本中，有5对在每对肿瘤中鉴定出完全相同的HBV DNA整合情况。7个CSC生物标志物在原发性或复发性肿瘤组织中的表达均显著增加。
结论
复发性HCC大多起源于其原发性肿瘤。DNA甲基化模式分析可为确定复发性HCC的起源提供一种新方法。
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