Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Division of Renewable Energy Engineering, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
Department of Civil Engineering, Inha University, 100 Inharo, Nam-gu, Incheon 402-751, Republic of Korea.
Bioresour Technol. 2015 Jun;186:215-222. doi: 10.1016/j.biortech.2015.03.031. Epub 2015 Mar 13.
In the study, at first, batch tests were performed to investigate the effect of alkali-shock on H2 production from food waste (FW). After alkali-pretreatment of FW at pH 9.0-13.0, the FW was cultivated under mesophilic condition at pH 6.0 for 30 h without external inoculum addition. The amount of H2 production from FW pretreated at pH 11.0 and 12.0 was higher than that achieved in other pretreatment pH. The main metabolite was butyrate, and Clostridium were dominant at pH 11.0 and 12.0. Meanwhile, lactate was the main metabolite with Enterococcus and Streptococcus being the dominant genus at other pretreatment pH. When the batch process was switched to a continuous mode, H2 production was significantly dropped due to the increased activity of H2-consumers. The reliability of alkali-pretreatment at pH 11.0 was proven by repeating the scale-up batch process, recording 1.57±0.11 mol H2/mol hexose(added) (17±2LH2/kg FW) and 4.39±0.32LH2/L/d.
在研究中，首先进行了分批试验，以研究碱冲击对食品废物（FW）产氢的影响。在 FW 的 pH 值为 9.0-13.0 进行碱预处理后，在没有外加接种物的情况下，在中温条件下培养 FW 30 小时，pH 值为 6.0。在 pH 值为 11.0 和 12.0 预处理的 FW 中产生的 H2 量高于其他预处理 pH 值下的 H2 产量。主要代谢产物为丁酸，pH 值为 11.0 和 12.0 时，以梭菌属为主。同时，在其他预处理 pH 值下，主要代谢产物为乳酸，优势属为肠球菌属和链球菌属。当分批过程切换到连续模式时，由于 H2 消耗菌活性的增加，H2 产量显著下降。通过重复放大批量过程证明了 pH 值为 11.0 的碱预处理的可靠性，记录了 1.57±0.11 mol H2/mol 己糖（添加）（17±2LH2/kg FW）和 4.39±0.32LH2/L/d。
