Department of Natural Resources and Environmental Engineering, Hanyang University, Seoul 133-791, South Korea.
School of Chemical and Biomolecular Engineering, Pusan National University, Busan 609-735, South Korea.
Bioresour Technol. 2015 Jun;186:232-237. doi: 10.1016/j.biortech.2015.03.078. Epub 2015 Mar 19.
A novel application of acid mine drainage (AMD) for biomass recovery of two morphologically different microalgae species with respect to AMD dosage, microalgal cell density and pH of medium was investigated. Optimal flocculation of Scenedesmus obliquus and Chlorella vulgaris occurred with 10% dosage of AMD at an initial pH 9 for both 0.5 and 1.0 g/L cell density. The flocculation efficiency was 89% for S. obliquus and 93% for C. vulgaris. Zeta potential (ZP) was increased from -10.66 to 1.77 and -13.19 to 1.33 for S. obliquus and C. vulgaris, respectively. Scanning electron microscope with energy-dispersive X-ray of the microalgae floc confirmed the sweeping floc formation mechanism upon the addition of AMD. Application of AMD for the recovery of microalgae biomass is a cost-effective method, which might further allow reuse of flocculated medium for algal cultivation, thereby contributing to the economic production of biofuel from microalgal biomass.
研究了酸性矿山排水 (AMD) 在不同剂量、不同藻细胞密度和不同介质 pH 值条件下对两种形态不同的微藻生物质回收的新应用。对于初始 pH 值为 9 的 0.5 和 1.0 g/L 藻细胞密度，10% AMD 剂量下可实现斜生栅藻和普通小球藻的最佳絮凝。斜生栅藻和普通小球藻的絮凝效率分别为 89%和 93%。Zeta 电位 (ZP) 分别从-10.66 增加到 1.77 和-13.19 增加到 1.33。微藻絮体的扫描电子显微镜和能量色散 X 射线证实了在添加 AMD 时的扫流絮体形成机制。AMD 用于回收微藻生物质是一种具有成本效益的方法，这可能进一步允许再利用絮凝后的培养基进行藻类培养，从而有助于从微藻生物质中经济地生产生物燃料。
