Gall J A, Bhathal P S
Cell Biol Int Rep. 1985 Apr;9(4):315-22. doi: 10.1016/0309-1651(85)90026-8.
A method is described for the isolation of biliary epithelial cells from rat livers by sequential treatment with EGTA, collagenase-hyaluronidase, trypsin, and deoxyribonuclease I and final separation on a discontinuous Percoll gradient using prekeratin antibodies as an immunohistochemical marker for these cells.
