Shao H-Z, Qin B-Y
Henan Provincial People's Hospital Zhengzhou China.
Henan Provincial People's Hospital Zhengzhou China qinbyu424@126.com.
Cell Mol Biol (Noisy-le-grand). 2015 Feb 28;61(1):1-6.
The binding of selectin to P—selectin glycoprotein ligand—1 (PSGL—1) mediates the tethering and rolling of leukocytes on the endothelium during leukocyte migration and inflammation. Recombinant human PSGL—1—Ig fusion protein (rPSGL—1—Ig) is a widely used selectin inhibitor that prevents neutrophil entry into inflamed or reperfused tissues. We hypothesized that rPSGL—1—Ig could be used to as a drug for the treatment of acute lung injury (ALI). We induced murine ALI by injecting mice with lipopolysaccharide (LPS) and then treated the mice with rPSGL—1—Ig. We determined the lung injury index, wet/dry ratio, and inflammatory cytokine level in differentially treated mice. The symptoms of LPS—induced lung injury were alleviated by rPSGL—1—Ig treatment. The histopathological index of LPS—induced lung injury improved after rPSGL—1—Ig treatment. rPSGL—1—Ig treatment also reduced the recruitment of inflammatory cells, including neutrophils, into the lung, as well as reducing the level of inflammatory cytokines. These data suggest that rPSGL—1—Ig protein has a therapeutic effect on LPS—induced lung injury.
选择素与P-选择素糖蛋白配体-1（PSGL-1）的结合介导白细胞迁移和炎症过程中白细胞在内皮细胞上的 tethering 和滚动。重组人PSGL-1-Ig融合蛋白（rPSGL-1-Ig）是一种广泛使用的选择素抑制剂，可阻止中性粒细胞进入发炎或再灌注组织。我们假设rPSGL-1-Ig可作为治疗急性肺损伤（ALI）的药物。我们通过给小鼠注射脂多糖（LPS）诱导小鼠ALI，然后用rPSGL-1-Ig治疗小鼠。我们测定了不同处理小鼠的肺损伤指数、湿/干比和炎性细胞因子水平。rPSGL-1-Ig治疗减轻了LPS诱导的肺损伤症状。rPSGL-1-Ig治疗后，LPS诱导的肺损伤组织病理学指数有所改善。rPSGL-1-Ig治疗还减少了包括中性粒细胞在内的炎性细胞向肺内的募集，同时降低了炎性细胞因子水平。这些数据表明，rPSGL-1-Ig蛋白对LPS诱导的肺损伤具有治疗作用。 （注：“tethering”此处可能是专业术语，暂未准确对应到合适中文，保留原文）
