Department of Civil and Environmental Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Clean Fuel Department, Korea Institute of Energy Research (KIER), 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
Bioresour Technol. 2015 Sep;191:469-74. doi: 10.1016/j.biortech.2015.03.020. Epub 2015 Mar 12.
In this study, a simultaneous process of harvesting biomass and extracting crude bio-oil was attempted from wet microalgae biomass using FeCl3 and Fe2(SO4)3 as both coagulant and cell-disrupting agent. A culture solution of Chlorella sp. KR-1 was firstly concentrated to 20 g/L and then proceeded for cell disruption with the addition of H2O2. Optimal dosage were 560 and 1060 mg/L for FeCl3 and Fe2(SO4)3, showing harvesting efficiencies of more than 99%. Optimal extraction conditions were identified via the response surface method (RSM), and the extraction yield was almost the same at 120 °C for both iron salts but FAME compositions after transesterification was found to be quite different. Given iron salts were a reference coagulant in water treatment in general and microalgae harvesting in particular, the present approach of using it for harvesting and oil-extraction in a simultaneous manner can serve as a practical route for the microalgae-derived biodiesel production.
在这项研究中，尝试使用 FeCl3 和 Fe2(SO4)3 作为混凝剂和细胞破碎剂，从湿微藻生物质中同时收获生物质和提取粗生物油。首先将小球藻 KR-1 的培养液浓缩至 20 g/L，然后加入 H2O2 进行细胞破碎。FeCl3 和 Fe2(SO4)3 的最佳剂量分别为 560 和 1060 mg/L，收获效率均超过 99%。通过响应面法（RSM）确定了最佳提取条件，两种铁盐在 120°C 下的提取产率几乎相同，但酯交换后的 FAME 组成却大不相同。鉴于铁盐通常是水处理中的参考混凝剂，特别是在微藻收获中，本研究中同时使用铁盐进行收获和油提取的方法可以作为微藻衍生生物柴油生产的实用途径。
