针对细胞色素P-450的单克隆抗体从人淋巴母细胞系中免疫纯化出一种45 kDa的蛋白质。
Monoclonal antibodies to cytochrome P-450 immunopurify a 45-kDa protein from a human lymphoblastoid cell line.
作者信息
Friedman F K, Pastewka J V, Robinson R C, Park S S, Marletta M A, Gelboin H V
出版信息
FEBS Lett. 1985 Jun 3;185(1):67-70. doi: 10.1016/0014-5793(85)80742-0.
Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.
针对大鼠肝细胞色素P-450的单克隆抗体（MAb）此前已成功用于从动物组织中免疫纯化细胞色素P-450。我们现在报告将这种基于单克隆抗体的免疫纯化技术应用于人类淋巴母细胞AHH-1细胞系。用3种不同的单克隆抗体进行免疫纯化，每种都产生了一种45 kDa的多肽。纯化后的蛋白质含有细胞色素P-450上存在的单克隆抗体特异性表位，因此可能是一种人类细胞色素P-450。
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