Bousfield G R, Liu W K, Ward D N
Mol Cell Endocrinol. 1985 Apr;40(1):69-77. doi: 10.1016/0303-7207(85)90159-5.
LH hybrids were prepared by combining eLH alpha and eLH beta with the corresponding subunits of oLH, pLH and hCG. Recombinants were isolated by gel filtration and assessed by SDS-polyacrylamide gel electrophoresis under both dissociating and non-dissociating conditions. All combinations of subunits produced hybrid LH molecules. Hybrids prepared by combining eLH beta with oLH alpha, pLH alpha or hCG alpha were very inactive in rat radioligand and Leydig cell in vitro bioassays. Hybrids prepared with eLH alpha were very active in both assays. The greatest potentiating activity was observed when eLH alpha was combined with pLH beta. The resulting hybrid was 49 times as active as pLH in stimulating steroidogenesis by Leydig cells.
通过将eLHα和eLHβ与oLH、pLH和hCG的相应亚基组合来制备LH杂种。通过凝胶过滤分离重组体，并在解离和非解离条件下通过SDS-聚丙烯酰胺凝胶电泳进行评估。亚基的所有组合都产生了杂种LH分子。通过将eLHβ与oLHα、pLHα或hCGα组合制备的杂种在大鼠放射性配体和Leydig细胞体外生物测定中非常无活性。用eLHα制备的杂种在两种测定中都非常活跃。当eLHα与pLHβ组合时观察到最大的增强活性。所得杂种在刺激Leydig细胞类固醇生成方面的活性是pLH的49倍。
