Wang Lingzhi, Huang Huansen, Wu Zhikun, Xia Zhengyuan, Liao Min
Department of Anesthesia, Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Mar;35(3):387-9, 396.
To examine the effect of acute incisional pain on the expression of connexin 43 in rat spinal cord dorsal horn.
Eighty rats were assigned into control group without any treatment and incisional pain group with incision surgery. For paw incisions, a 1-cm longitudinal incision was made through the skin and fascia of the plantar aspect of the right hind paw. After surgery, the 50% paw withdrawal threshold (PWT) was assessed in response to a tactile stimulus with calibrated von Frey monofilaments at 1, 2, 4 and 24 h, respectively. The spinal cord dorsal horn of rats was isolated at 1, 2, and 4 h after the surgery to assess the expression of connexin 43 using Western blotting and immunofluorescence assay.
The 50% PWT of the rats was significantly decreased after the incision surgery, and this decrement was the most obvious at 2 and 4 h. Western blotting and immunofluorescence assay showed that the expression of connexin 43 in the spinal cord dorsal horn was significantly increased in rats receiving the surgery especially at 2 and 4 h after the surgery.
Incision surgery induces an significant increase in connexin 43 expression in rat spinal cord dorsal horn, suggestting an potential role of connexin43 in postoperative incisional pain.
研究急性切口疼痛对大鼠脊髓背角中连接蛋白43表达的影响。
将80只大鼠分为未接受任何处理的对照组和接受切口手术的切口疼痛组。对于后爪切口，在右后爪足底侧的皮肤和筋膜上做一个1厘米的纵向切口。术后分别在1、2、4和24小时，用校准的von Frey单丝对触觉刺激进行评估，测定50% paw withdrawal threshold（PWT）。术后1、2和4小时分离大鼠脊髓背角，采用蛋白质免疫印迹法和免疫荧光法评估连接蛋白43的表达。
切口手术后大鼠的50% PWT显著降低，在2和4小时时这种降低最为明显。蛋白质免疫印迹法和免疫荧光法显示，接受手术的大鼠脊髓背角中连接蛋白43的表达显著增加，尤其是在术后2和4小时。
切口手术导致大鼠脊髓背角中连接蛋白43的表达显著增加，提示连接蛋白43在术后切口疼痛中可能发挥作用。
