Piroird Cécile, Ovigne Jean-Marc, Rousset Françoise, Martinozzi-Teissier Silvia, Gomes Charles, Cotovio José, Alépée Nathalie
L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
L'Oréal Research & Innovation, 1, Avenue Eugène Schueller, 93600 Aulnay-sous-Bois Cedex, France.
Toxicol In Vitro. 2015 Aug;29(5):901-16. doi: 10.1016/j.tiv.2015.03.009. Epub 2015 Mar 24.
The U-SENS™ assay, formerly known as MUSST (Myeloid U937 Skin Sensitization Test), is an in vitro method to assess skin sensitization. Dendritic cell activation following exposure to sensitizers was modelled in the U937 human myeloid cell line by measuring the induction of the expression of CD86 by flow cytometry. The predictive performance of U-SENS™ was assessed via a comprehensive comparison analysis with the available human and LLNA data of 175 substances. U-SENS™ showed 79% specificity, 90% sensitivity and 88% accuracy. A four laboratory ring study demonstrated the transferability, reliability and reproducibility of U-SENS™, with a reproducibility of 95% within laboratories and 79% between-laboratories, showing that the U-SENS™ assay is a promising tool in a skin sensitization risk assessment testing strategy.
U-SENS™检测法，前身为MUSST（髓系U937皮肤致敏试验），是一种评估皮肤致敏性的体外方法。通过流式细胞术测量致敏剂暴露后U937人髓系细胞系中树突状细胞的活化情况，以CD86表达的诱导来进行建模。通过与175种物质的现有人类数据和LLNA数据进行全面比较分析，评估了U-SENS™的预测性能。U-SENS™显示出79%的特异性、90%的敏感性和88%的准确性。一项四实验室环行研究证明了U-SENS™的可转移性、可靠性和可重复性，实验室内部的可重复性为95%，实验室之间为79%，表明U-SENS™检测法是皮肤致敏风险评估测试策略中一种很有前景的工具。
