Clayberger C, Uyehara T, Hardy B, Eaton J, Karasek M, Krensky A M
J Immunol. 1985 Jul;135(1):12-8.
Two long-term cytolytic T lymphocyte (CTL) lines derived from the peripheral blood lymphocytes (PBL) of a single donor were analyzed for target specificity and involvement of cell surface molecules in CTL-target interactions. One line, AH2, was generated after stimulation with B lymphoblastoid cells. Cytolysis by these cells was restricted to targets expressing the appropriate HLA-A2 specificity and was blocked by mAb recognizing CD2, CD3, CD8, LFA-1, and LFA-3. The second line, AE1, was generated after stimulation with cultured endothelial cells derived from human newborn preputial microvessels. These CTL lysed all human target cells tested, except autologous cells and the Class I negative cell line Daudi. In addition, mAb specific for CD2, CD3, and CD8 did not affect cytolysis. Anti-LFA-1 and -LFA-3 mAb blocked cytolysis of B lymphoblastoid targets but not endothelial targets. These results indicate that some CTL utilize as yet uncharacterized cell surface structures for CTL-target interactions.
对从一名供体的外周血淋巴细胞（PBL）中获得的两条长期细胞毒性T淋巴细胞（CTL）系进行了分析，以研究其靶标特异性以及细胞表面分子在CTL与靶标相互作用中的作用。其中一条系AH2是在用B淋巴母细胞刺激后产生的。这些细胞的细胞溶解作用仅限于表达适当HLA - A2特异性的靶标，并且被识别CD2、CD3、CD8、LFA - 1和LFA - 3的单克隆抗体所阻断。第二条系AE1是在用源自人类新生儿包皮微血管的培养内皮细胞刺激后产生的。这些CTL能溶解所有测试的人类靶细胞，但自体细胞和I类阴性细胞系Daudi除外。此外，针对CD2、CD3和CD8的单克隆抗体不影响细胞溶解作用。抗LFA - 1和 - LFA - 3单克隆抗体可阻断B淋巴母细胞靶标的细胞溶解作用，但不影响内皮靶标的细胞溶解作用。这些结果表明，一些CTL利用尚未明确的细胞表面结构进行CTL与靶标的相互作用。
